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荧光素系列染料
Release Time：2022-03-05荧光素系列染料介绍：
荧光素衍生物具有高吸收率、优异的荧光量子产率和良好的水溶性，是流式细胞仪和免疫荧光中生物检测最常用的荧光标记之一。最常用的荧光素包括用于标记蛋白质（特别是抗体）的异硫氰酸荧光素 [5(6)-FITC)]，5-FITC 和用于标记肽和寡核苷酸的羧基荧光素（5-FAM 和 5(6)-FAM）。 BIOFOUNT 提供广泛的基于荧光素的染料、底物和偶联物，以及一系列具有针对细胞标记和检测优化的特性的优质 iFluor™ 荧光标记染料。
荧光素光谱特性：
表 1. 5-异硫氰酸荧光素 (5-FITC) 的光谱特性。
| 参数 | 值 |
| Ex/Em (nm): | 494/525 nm |
| Extinction Coefficient (ε): | 75,000 cm-1M-1 |
| Filter Set: | FITC |
| Laser Line: | 488 nm laser |
| Quantum Yield (Φ): | 0.79 |
※ 5-异硫氰酸荧光素 (5-FITC) 的激发和发射光谱
荧光素活性染料：
反应性荧光荧光素和荧光素衍生物广泛用于共价标记生物分子，如抗体、碳水化合物、核酸、蛋白质、肽和寡核苷酸。 荧光素偶联物产生强烈明亮且光稳定的绿色荧光，通常用作检测酶活性的底物，或在与蓝色、黄色或红色荧光探针结合时在多色荧光研究中提供对比。 荧光素和荧光素衍生物很容易在各种染料标记化学物质中获得，包括：
琥珀酰亚胺酯/NHS 酯 - 用于标记抗体、蛋白质、肽、胺修饰寡核苷酸和其他生物分子上的游离胺 (-NH2)
※ 马来酰亚胺 - 用于标记抗体、蛋白质和肽上的巯基
※ 叠氮化物 - 用于通过点击化学方法标记亚乙基
※ 炔烃 - 通过点击化学方法标记叠氮化物
※ 酰肼 - 用于标记醛和酮基团
※ 羧酸 - 用于在碳二亚胺预活化后标记胺或用于醇的 Steglich 酯化
※ 胺 - 用于标记各种亲电子化合物，例如活化酯
※ 四嗪 - 用于通过逆电子需求 Diels-Alder 反应标记反式环辛烯 (TCO) 化合物
※ 亚磷酰胺 - 用于标记寡核苷酸
表 2. 可用于标记生物分子的反应性荧光素和荧光素衍生物。
| 产品 | 反应基团 | 活性基团 | 吸收波长 (nm) | 发射波长(nm) | 消光系数(εmax) | 规格 | Cat No. |
| 3'-Fluorescein CPG | Oligonucleotide 3' end labeling | 487 | 517 | 80000 | |||
| 3'-(6-Fluorescein) CPG | Oligonucleotide 3' end labeling | 487 | 517 | 80000 | |||
| 5(6)-FAM, SE *CAS 117548-22-8* | Amine | Succinimidyl Ester | 493 | 517 | 83000 | ||
| 5(6)-FAM *CAS 72088-94-9* | Amines after pre-activation with carbodiimides | Carboxylic Acid | 493 | 517 | 83000 | ||
| 5(6)-FAM ethylenediamine | Aldehyde, Carboxylic Acid, Ketone | Ethylenediamine | 493 | 517 | 83000 | ||
| 5-FAM, SE | Amine | Succinimidyl Ester | 493 | 517 | 83000 | ||
| 5-FAM-X, SE *CAS 148356-00-7* | Amine | Succinimidyl Ester | 493 | 517 | 83000 | ||
| 5-FAM Azide | Alkyne | Azide | 493 | 517 | 83000 | ||
| 5-FAM Alkyne | Azide | Alkyne | 493 | 517 | 83000 | ||
| 5-FAM *CAS 76823-03-5* | Amines after pre-activation with carbodiimides | Carboxylic Acid | 493 | 517 | 83000 | ||
| 5-FAM ethylenediamine | Aldehyde, Carboxylic Acid, Ketone | Ethylenediamine | 493 | 517 | 83000 | ||
| 5-DTAF *CAS 51306-35-5* | Amines and Thiols | Dichlorotriazine | 498 | 517 | 80000 | ||
| 6-FAM, SE *CAS 92557-81-8* | Amine | Succinimidyl Ester | 493 | 517 | 83000 | ||
| 6-FAM Azide | Alkyne | Azide | 493 | 517 | 83000 | ||
| 6-FAM Alkyne | Azide | Alkyne | 493 | 517 | 83000 | ||
| 6-FAM *CAS 3301-79-9* | Amines after pre-activation with carbodiimides | Carboxylic Acid | 493 | 517 | 83000 | ||
| 6-FAM phosphoramidite *CAS 204697-37-0* | Oligonucleotide 5' end labeling | Phosphoramidite | 493 | 517 | 83000 | ||
| OG488 maleimide | Thiol | Maleimide | 498 | 526 | 76000 | ||
| 5-OG488 succinimidyl ester | Amine | Succinimidyl Ester | 498 | 526 | 76000 | ||
| 5-OG488 carboxylic acid | Amines after pre-activation with carbodiimides | Carboxylic Acid | 498 | 526 | 76000 | ||
| 6-OG488 succinimidyl ester | Amine | Succinimidyl Ester | 498 | 526 | 76000 | ||
| 6-OG488 carboxylic acid | Amines after pre-activation with carbodiimides | Carboxylic Acid | 498 | 526 | 76000 | ||
| 6-TET, SE | Amine | Succinimidyl Ester | 521 | 542 | 73000 | ||
| 6-TET azide | Alkyne | Azide | 521 | 542 | 73000 | ||
| 6-TET alkyne | Azide | Alkyne | 521 | 542 | 73000 | ||
| 6-TET phosphoramidite *CAS 877049-90-6* | Oligonucleotide 5' end labeling | Phosphoramidite | 521 | 542 | 73000 | ||
| 6-JOE, SE | Amine | Succinimidyl Ester | 520 | 545 | 75000 | ||
| 6-JOE, SE | Amine | Succinimidyl Ester | 520 | 545 | 75000 | ||
| 6-JOE azide | Alkyne | Azide | 520 | 545 | 75000 | ||
| 6-JOE Phosphoramidite | Oligonucleotide 5' end labeling | Phosphoramidite | 520 | 545 | 75000 | ||
| 6-HEX, SE *Single Isomer* | Amine | Succinimidyl Ester | 533 | 559 | 73000 | ||
| 6-HEX azide | Alkyne | Azide | 533 | 559 | 73000 | ||
| 6-HEX alkyne | Azide | Alkyne | 533 | 559 | 73000 | ||
| 6-HEX phosphoramidite | Oligonucleotide 5' end labeling | Phosphoramidite | 533 | 559 | 73000 | ||
| FITC-xtra | Amine | Succinimidyl Ester | 498 | 526 | 73000 | ||
| 5-FITC isothiocyanate *CAS 3326-32-7* | Amine | Isothiocyanate | 491 | 516 | 73000 | ||
| 5-IAF [5-Iodoacetamidofluorescein] | Thiol | Iodoacetamide | 498 | 517 | 80000 | ||
| Fluorescein-5-maleimide | Thiol | Maleimide | 498 | 517 | 80000 | ||
| 5-FITC cadaverine | Labels proteins by transaminidation | Cadaverine | 491 | 516 | 73000 | ||
| 5-FITC ethylenediamine | Aldehyde, Carboxylic Acid, Ketone | Ethylenediamine | 491 | 516 | 73000 | ||
| 6-Fluorescein phosphoramidite | Oligonucleotide 5' end labeling | Phosphoramidite | 498 | 517 | 80000 | ||
| AMF [4'-(Aminomethyl)fluorescein] | Activated Carboxy Esters (NHS Esters) | Amino | 487 | 517 | 80000 | ||
| Tide Fluor™ 2 alkyne | Azide | Alkyne | 503 | 525 | 75000 | ||
| Tide Fluor™ 2 amine | Carbonyls | Amine | 503 | 525 | 75000 | ||
| Tide Fluor™ 2 azide | Alkyne | Azide | 503 | 525 | 75000 | ||
| Tide Fluor™ 2 carboxylic acid | Amines after pre-activation with carbodiimides | Carboxylic Acid | 503 | 525 | 75000 | ||
| Tide Fluor™ 2 maleimide | Thiol | Maleimide | 503 | 525 | 75000 | ||
| Tide Fluor™ 2 succinimidyl ester | Amine | Succinimidyl Ester | 503 | 525 | 75000 | ||
| Tide Fluor™ 2WS amine | Carbonyls | Amine | 503 | 525 | 75000 | ||
| Tide Fluor™ 2WS carboxylic acid | Amines after pre-activation with carbodiimides | Carboxylic Acid | 503 | 525 | 75000 | ||
| Tide Fluor™ 2WS maleimide | Thiol | Maleimide | 503 | 525 | 75000 | ||
| Tide Fluor™ 2WS succinimidyl ester | Amine | Succinimidyl Ester | 503 | 525 | 75000 |
荧光素标记的酶底物：
荧光素和荧光素衍生物经常用于制备荧光底物，以检测细胞和溶液中的酶活性。 当被感兴趣的酶水解时，荧光素修饰的底物表现出与酶活性成线性比例的荧光强度增加。 BIOFOUNT 提供种类繁多的荧光素修饰底物，用于检测半胱天冬酶、蛋白酶、肽酶、磷酸酶等的活性。
表 3. 荧光素标记的荧光底物。
| 产品 | 生物功能 | Ex (nm) | Em (nm) | 规格 | 货号 |
| Casein, FITC-conjugated | Generic Protease Substrate | 498 | 517 | 5 mg | |
| FAM-VAD-FMK | Polycaspase Inhibitor (Caspases 1, 2, 3, 6, 8, 9, or 10) | 493 | 517 | 25 Tests | |
| FCB [Fluorescein di-beta-D-cellobioside] | Cellulase (e.g. β-glucosidases, endoglucanases and exoglucanases) Substrate | 498 | 517 | 1 mg | |
| FDG [Fluorescein di-beta-D-galactopyranoside] | β-galactosidase substrate | 498 | 517 | 1 g | |
| FDGlcU [Fluorescein di-beta-D-glucuronide] | β-Glucuronidase Substrate | 498 | 517 | 1 mg | |
| FDP [Fluorescein diphosphate, tetraammonium salt] *CAS 217305-49-2* | Phosphatase Substrate | 498 | 517 | 5 mg | |
| FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK | Caspase 3 Substrate | 491 | 516 | 100 µg | |
| FITC-C6-DEVD-FMK | Caspase 3 Substrate | 491 | 516 | 100 µg | |
| FITC-C6-LEHD-FMK | Caspase 9 Substrate | 491 | 516 | 100 µg | |
| Glucose-UDP-(PEG)6-Fluorescein Conjugate | Glucosyltransferase Substrate | 498 | 517 | 100 µg | |
| Glucose-UDP-Fluorescein Conjugate | Glucosyltransferase Substrate | 498 | 517 | 100 µg |
荧光素指示剂
荧光素衍生指示剂是生化和细胞研究的重要工具。 它们已用于所有主要类型的荧光成像应用，包括流式细胞术、荧光显微镜和基于荧光板的分析。 BIOFOUNT 提供范围广泛的荧光素衍生指标，用于测量细胞 pH 值、钙浓度、细胞增殖、细胞活力等。
表 6. 荧光素衍生指示剂产品
| 产品 | 生物功能 | Ex (nm) | Em (nm) | 规格 | 货号 |
| BCECF acid *CAS#: 85138-49-4* | pH Indicator | 504 | 527 | 1 mg | |
| BCECF, AM | Cell-Permeable pH Indicator | 504 | 527 | 1 mg | |
| CDCFDA [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate] *Mixed isomers* | Cell Viability Indicator | 498 | 517 | 100 mg | |
| CDCFDA, SE [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester] *Mixed isomers* | Cell Proliferation Indicator, Cell Viability Indicator | 498 | 517 | 25 mg | |
| CFDA [5-(and 6)-Carboxyfluorescein diacetate] *Mixed isomers* | Cell Viability Indicator | 498 | 517 | 100 mg | |
| CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4* | Cell Proliferation Indicator, Cell Viability Indicator | 498 | 517 | 25 mg | |
| CytoFix™ BCECF, AM *Optimized for long term cellular pH tracking* | Cell-Permeable and Fixable pH Indicator | 504 | 527 | 1 mg | |
| Dihydrofluorescein diacetate [Fluorescin diacetate] *CAS 35340-49-9* | Cell Viability Indicator, Redox Indicator | 498 | 517 | 100 mg | |
| FDA [Fluorescein diacetate] *CAS 596-09-8* | Cell Viability Indicator | 498 | 517 | 1 g | |
| Fluo-2, AM | Cell-Permeable Calcium Indicator (Kd = 232 nM) | 488 | 515 | 1 mg | |
| Fluo-2, potassium salt | Cell-Impermeable Calcium Indicator (Kd = 232 nM) | 488 | 515 | 10 x 50 µg | |
| Fluo-3, AM | Cell-Permeable Calcium Indicator (Kd = 390 nM) | 507 | 516 | 1 mg | |
| Fluo-3, pentaammonium salt | Cell-Impermeable Calcium Indicator (Kd = 390 nM) | 507 | 516 | 1 mg | |
| Fluo-3, pentapotassium salt | Cell-Impermeable Calcium Indicator (Kd = 390 nM) | 507 | 516 | 1 mg | |
| Fluo-3, pentasodium salt | Cell-Impermeable Calcium Indicator (Kd = 390 nM) | 507 | 516 | 1 mg | |
| Fluo-4 AM | Cell-Permeable Calcium Indicator (Kd = 345 nM) | 495 | 528 | 1 mg | |
| Fluo-4, Pentapotassium Salt | Cell-Impermeable Calcium Indicator (Kd = 345 nM) | 495 | 528 | 1 mg | |
| Fluo-5F, AM | Cell-Permeable Calcium Indicator (Kd = 2.3 µM) | 494 | 516 | 10 x 50 µg | |
| Fluo-5F, pentapotassium Salt | Cell-Impermeable Calcium Indicator (Kd = 2.3 µM) | 494 | 516 | 500 µg | |
| Fluo-5N, AM | Cell-Permeable Calcium Indicator (Kd = 90 µM) | 494 | 516 | 10 x 50 µg | |
| Fluo-5N, pentapotassium Salt | Cell-Impermeable Calcium Indicator (Kd = 90 µM) | 494 | 516 | 500 µg | |
| Fluo-8FF™, AM | Cell-Impermeable Calcium Indicator (Kd = 10 µM) | 495 | 516 | 1 mg | |
| Fluo-8FF™, potassium salt | Cell-Impermeable Calcium Indicator (Kd = 10 µM) | 495 | 516 | 1 mg | |
| Fluo-8H™, AM | Cell-Impermeable Calcium Indicator (Kd = 232 nM) | 495 | 516 | 1 mg | |
| Fluo-8H™, sodium salt | Cell-Impermeable Calcium Indicator (Kd = 232 nM) | 495 | 516 | 10 x 50 µg | |
| Fluo-8L™, AM | Cell-Impermeable Calcium Indicator (Kd = 1.9 µM) | 495 | 516 | 1 mg | |
| Fluo-8L™, potassium salt | Cell-Impermeable Calcium Indicator (Kd = 1.9 µM) | 495 | 516 | 1 mg | |
| Fluo-8L™, sodium salt | Cell-Impermeable Calcium Indicator (Kd = 1.9 µM) | 495 | 516 | 1 mg | |
| Fluo-8®, AM | Cell-Permeable Calcium Indicator (Kd = 389 nM) | 495 | 516 | 1 mg | |
| Fluo-8®, potassium salt | Cell-Impermeable Calcium Indicator (Kd = 389 nM) | 495 | 516 | 1 mg | |
| Fluo-8®, sodium salt | Cell-Impermeable Calcium Indicator (Kd = 389 nM) | 495 | 516 | 1 mg | |
| Fluorescein dicaprylate *Water-Soluble* | Cell Viability Indicator | 498 | 517 | 5 mg | |
| Fluorescein dicaprylate [Fluorescein dioctanoate] | Cell Viability Indicator | 498 | 517 | 100 mg | |
| Mag-Fluo-4 AM | Cell-Permeable Mg Indicator (Kd = 4.7 mM), Low-Affinity Calcium Indicator (Kd = 22 µM) | 495 | 528 | 10 x 50 µg | |
| Mag-Fluo-4 potassium salt | Cell-Permeable Mg Indicator (Kd = 4.7 mM), Low-Affinity Calcium Indicator (Kd = 22 µM) | 495 | 528 | 10 x 50 µg | |
| RatioWorks™ BCFL, AM | Cell-Permeable pH Indicator | 503 | 524 | 1 mg | |
| ReadiUse™ CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4* | Cell Proliferation Indicator, Cell Viability Indicator | 498 | 517 | 5 x 500 µg |
