65358-15-8|伪胸苷|Pseudothymidine|MFCD04973713-范德生物科技公司

伪胸苷

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names：
Pseudothymidine
CAS号：
65358-15-8
MDL Number： MFCD04973713
MF(分子式)： C10H14N2O5 MW(分子量)： 242.23
EINECS:No data available Reaxys Number:No data available
Pubchem ID:375973985 Brand:BIOFOUNT

伪胸苷(65358-15-8,Pseudothymidine)是胸苷的C-核苷（C-nucleosid）类似物。

货品编码	规格	纯度	价格 (¥)	现价(¥)	特价(¥)	库存描述	数量	总计 (¥)
YZM000669-5mg	5mg	99.85%	¥ 6581.00	¥ 6581.00		2-3天	- +	¥ 0.00
YZM000669-1mg	1mg	99.85%	¥ 1852.50	¥ 1852.50		2-3天	- +	¥ 0.00

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询价

中文别名	伪胸苷(65358-15-8);5-(2-脱氧-BETA-D-赤式-呋喃戊糖基)-1-甲基-2,4(1H,3H)-嘧啶二酮;
英文别名	Pseudothymidine(65358-15-8);5-Methyl-2'-Deoxypseudouridin;5-(2-Deoxy-beta-D-erythro-pentofuranosyl)-1-methyl-2,4(1H,3H)-pyrimidinedione;PSEUDOTHYMIDINE;5-Methyl-2'-Deoxypseudouridin;
CAS号	65358-15-8
SMILES	O=C(N1)C([C@@H]2O[C@H](CO)[C@@H](O)C2)=CN(C)C1=O
Inchi	No data available
InchiKey	No data available
分子式 Formula	C10H14N2O5
分子量 Molecular Weight	242.23
闪点 FP	No data available
熔点 Melting point	No data available
沸点 Boiling point	No data available
Polarizability极化度	No data available
密度 Density	1.452±0.06 g/cm3(Predicted)
蒸汽压 Vapor Pressure	No data available
溶解度Solubility	生物体外In Vitro:DMSO溶解度≥ 61.17 mg/mL(252.53 mM)*"≥" means soluble可溶, but saturation unknown溶解度未知.
性状	固体粉末,Power
储藏条件 Storage conditions	-20°C 3 years年 4°C 2 years年 / In solvent溶液中:-80°C 6 months月 -20°C 1 month月

伪胸苷(65358-15-8,Pseudothymidine)实验注意事项：
1.实验前需戴好防护眼镜，穿戴防护服和口罩，佩戴手套，避免与皮肤接触。
2.实验过程中如遇到有毒或者刺激性物质及有害物质产生，必要时实验操作需要手套箱内完成以免对实验人员造成伤害
3.实验后产生的废弃物需分类存储，并交于专业生物废气物处理公司处理，以免造成环境污染Experimental considerations:
1. Wear protective glasses, protective clothing and masks, gloves, and avoid contact with the skin during the experiment.
2. The waste generated after the experiment needs to be stored separately, and handed over to a professional biological waste gas treatment company to avoid environmental pollution.

Tags:Pseudothymidine试剂,Pseudothymidine杂质,Pseudothymidine中间体,Pseudothymidine密度,Pseudothymidine旋光度,Pseudothymidine溶解度,Pseudothymidine闪点,Pseudothymidine熔点,Pseudothymidine结构式,Pseudothymidine购买,

产品说明	(65358-15-8,Pseudothymidine)伪胸苷是胸苷的C-核苷（C-nucleosid）类似物。
Introduction	(伪胸苷,65358-15-8)Pseudothymidine is a Cucleoside analog of thymidine.
Application1
Application2
Application3

伪胸苷(65358-15-8,Pseudothymidine)药理学:

伪胸苷是胸苷的C-核苷（C-nucleosid）类似物。

警示图
危险性	warning
危险性警示	Not available
安全声明	H303吞入可能有害+H313皮肤接触可能有害+H2413吸入可能对身体有害
安全防护	P264处理后彻底清洗+P280戴防护手套/穿防护服/戴防护眼罩/戴防护面具+P305如果进入眼睛+P351用水小心冲洗几分钟+P338取出隐形眼镜（如果有）并且易于操作,继续冲洗+P337如果眼睛刺激持续+P2393获得医疗建议/护理
备注	实验过程中防止吸入、食入，做好安全防护

S Lutz, et al. An in vitro screening technique for DNA polymerases that can incorporate modified nucleotides. Pseudo-thymidine as a substrate for thermostable polymerases. Nucleic Acids Res. 1999 Jul

Havemann SA, et al. Incorporation of multiple sequential pseudothymidines by DNA polymerases and their impact on DNA duplex structure. Nucleosides Nucleotides Nucleic Acids. 2008 Mar;27(3):261-78.

伪胸苷(65358-15-8,Pseudothymidine)参考文献：

1、Incorporation of multiple sequential pseudothymidines by DNA polymerases and their impact on DNA duplex structure
Stephanie A Havemann , Shuichi Hoshika, Daniel Hutter, Steven A Benner

Abstract：Thermal denaturation and circular dichroism studies suggested that multiple (up to 12), sequential pseudothymidines, a representative C-glycoside, do not perturb the structure of a representative DNA duplex. Further, various Family A and B DNA polymerases were found to extend a primer by incorporating four sequential pseudothymidine triphosphates, and then continue the extension to generate full-length product. Detailed studies showed that Taq polymerase incorporated up to five sequential C-glycosides, but not more. These results constrain architectures for sequencing, quantitating, and analyzing DNA analogs that exploit C-glycosides, and define better the challenge of creating a synthetic biology using these with natural polymerases.

2、An in vitro screening technique for DNA polymerases that can incorporate modified nucleotides. Pseudo-thymidine as a substrate for thermostable polymerases
S Lutz , P Burgstaller, S A Benner

Abstract：DNA polymerases are desired that incorporate modified nucleotides into DNA with diminished pausing, premature termination and infidelity. Reported here is a simple in vitro assay to screen for DNA polymerases that accept modified nucleotides based on a set of primer extension reactions. In combination with the scintillation proximity assay (SPA[trade]), this allows rapid and simple screening of enzymes for their ability to elongate oligonucleotides in the presence of unnatural nucleotides. A proof of the concept is obtained using pseudo-thymidine (psiT), the C-nucleoside analog of thymidine, as the unnatural substrate. The conformational properties of psiT arising from the carbon-carbon bond between the sugar and the base make it an interesting probe for the importance of conformational restraints in the active site of polymerases during primer elongation. From a pool of commercially available thermostable polymerases, the assay identified Taq DNA polymerase as the most suitable enzyme for the PCR amplification of oligonucleotides containing psiT. Subsequent experiments analyzing PCR performance and fidelity of Taq DNA polymerase acting on psiT are presented. This is the first time that PCR has been performed with a C-nucleoside.

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