MCA succinimidyl ester

---

MCA NHS Ester（AMCA NHS酯）介绍：
MCA NHS Ester（琥珀酰-7-氨基-4-甲基香豆素-3-乙酸）是最流行的蓝色荧光标记分子之一。MCA NHS Ester 广泛用于标记抗体、蛋白质和药物小分子。 通常用作免疫荧光显微镜、阵列和原位杂交中双重和三重标记的对比探针。 AMCA 染料的理想特性包括相对较大的斯托克位移和抗光漂白性。


AMCA NHS酯 在 pH 7-9 下与伯胺（例如，赖氨酸残基的侧链或氨基硅烷涂层表面）特异性且有效地反应，形成稳定的共价酰胺键。 NHS 酯（或琥珀酰亚胺酯）是将染料与蛋白质或抗体 (Lys) 的伯胺、胺修饰的寡核苷酸和其他含胺分子结合的最常用工具。

---

AMCA NHS Ester (succinimydyl-7-amino-4-methylcoumarin-3-acetic acid) is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. Often used as contrasting probes for double- and triple-labeling in immunofluorescence microscopy, arrays and in situ hybridization. The desirable properties of AMCA dyes include a relatively large Stoke’s shift and resistance to photobleaching.

AMCA NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

---

AMCA NHS Ester光谱图

---