1345980-40-6|Fluo-8, AM钙离子荧光探针|CalciFluor Fluo-8, AM|-范德生物科技公司

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Fluo-8, AM钙离子荧光探针
NMR and HPLC COA下载 MSDS下载
Names：
CalciFluor Fluo-8, AM
CAS号：
1345980-40-6
MDL Number：
MF(分子式)： C50H50N2O23 MW(分子量)： 1046.93
EINECS: Reaxys Number:
Pubchem ID:54575324 Brand:BIOFOUNT

Fluo-8, AM钙离子荧光探针;(CalciFluor™ Fluo-8, AM) 是一种绿色荧光钙结合染料，是一种优于传统 Fluo-4, AM 的 Ca2+ 荧光探针。荧光 : λex 494 nm, λem 517 nm in DMSO

货品编码	规格	纯度	价格 (¥)	现价(¥)	特价(¥)	库存描述	数量	总计 (¥)
HCM0029B-1mg	1mg	98%ab	¥ 0.00	¥ 0.00	4800	2-3weeks	- +	¥ 0.00

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中文别名	钙离子荧光探针Fluo-8, AM;钙离子荧光探针；钙荧光探针Fluo-8 AM；钙离子探针；钙荧光探针;钙荧光探针 Fluo-8 AM
英文别名	CalciFluor Fluo-8, AM；Fluo-8, AM；Fluo-8 AM；Bis(acetoxymethyl) 2,2'-((4-(6-(acetoxymethoxy)-3-oxo-3H-xanthen-9-yl)-2-(2-(bis(2-acetoxymethoxy)-2-oxoethyl)amino)phenoxy)ethoxy)phenyl)azanediyl)diacetate
CAS号	1345980-40-6
SMILES	CC(=O)OCOC1=CC2=C(C=C1)C(=C3C=CC(=O)C=C3O2)C4=CC(=C(C=C4)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC5=CC
Inchi	InChI=1S/C50H50N2O23/c1-30(53)65-25-70-37-12-14-39-44(20-37)75-43-19-36(58)11-13-38(43)50(39)35-10-15-41(52(23-48(61)73-28-68-33(4)56)24-49(62)74-29-69-34(5)57)45(18-35)64-17-16-63-42-9-7-6-8-40(42)51(21-46(59)71-26-66-31(2)54)22-47(60)72-27-67-32(3)55/h6-15,18-20H,16-17,21-29H2,1-5H3
InchiKey	BKYHTWFRZPRHNM-UHFFFAOYSA-N
分子式 Formula	C50H50N2O23
分子量 Molecular Weight	1046.93
闪点 FP	No data available
熔点 Melting point	No data available
沸点 Boiling point	No data available
Polarizability极化度
密度 Density	No data available
蒸汽压 Vapor Pressure
溶解度Solubility	Soluble in DMSO, and water (low).
性状	Solid power
储藏条件 Storage conditions	Desiccate at -20° C

Fluo-8, AM 钙离子荧光探针CalciFluor™ Fluo-8, AM 的应用
CalciFluor™ Fluo-8, AM 已开发用于改善细胞负载和钙响应，同时保持便利的 Fluo-3 和 Fluo-4 光谱波长，最大激发 @ ~490 nm 和最大发射 @ ~520 nm。与 Fluo 4 AM 类似，阴离子羧基被乙酰氧基甲酯保护，允许膜渗透，随后被细胞内酯酶去除 - 允许 Ca2+ 检测。

CalciFluor™ Fluo-8, AM has been developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation @ ~490 nm and maximum emission @ ~520 nm. Similarly to Fluo 4 AM, the anionic carboxy groups are protected with acetoxy methyl esters allowing membrane permeability, which are subsequently removed by intracellular esterases - allowing Ca2+ detection. However CalciFluor™ Fluo-8, AM is efficiently loaded into cells at room temperature (whereas Fluo-3 and Fluo-4 require much more harsh loading conditions), and CalciFluor™ Fluo-8, AM is 2 times brighter than Fluo-4 and 4 times brighter than Fluo-3. These features, as well as a lower price, make CalciFluor™ Fluo-8, AM a superior in vivo calcium indicator.

FLUO-8 (AM) 是 Fluo-8 的一种可渗透细胞的乙酰氧基甲基 (AM) 酯衍生物。亮度大约是 Fluo-4 的两倍。吸光度 452 nm (26,000)；发射 515 nm（AM 水解后；溶于 10 mM EGTA、100 mM KCl、10 mM MOPS，pH 7.2）

Fluo-8 AM, green fluorescent calcium binding dye -- 荧光细胞成像
U2OS 细胞在 96 孔黑壁/透明底部 costar 板中以每 100 µL/孔 40,000 个细胞过夜播种。去除生长培养基，将细胞与 100 µl 4 µM Fluo-4 AM 或 Fluo-8® AM 在 HHBS 中在 37 °C、5% CO2 培养箱中孵育 1 小时。细胞用 200 µl HHBS 洗涤两次，然后使用 FITC 通道在荧光显微镜下成像。

产品说明	Fluo-8, AM钙离子荧光探针;(CalciFluor Fluo-8, AM) 是一种绿色荧光钙结合染料，是一种优于传统 Fluo-4, AM 的 Ca2+ 荧光探针。荧光 : λex 494 nm, λem 517 nm in DMSO
Introduction	CalciFluor Fluo-8, AM is a green fluorescent calcium binding dye, a superior Ca2+ fluorescent probe than traditional Fluo-4, AM.
Application1
Application2
Application3

Calcium measurements are critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Fluo-3 AM and Fluo-4 AM are most commonly used among the visible light-excitable calcium indicators for live-cell calcium imaging. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis and require harsh cell loading conditions to maximize their cellular calcium responses. Fluo-8® dyes are developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelengths of Ex/Em = ∼490/∼520 nm. Fluo-8® AM can be loaded into cells at room temperature, while Fluo-3 AM and Fluo-4 AM require 37°C for cell loading. In addition, Fluo-8® AM is two times brighter than Fluo-4 AM and four times brighter than Fluo-3 AM. AAT Bioquest offers a set of our outstanding Fluo-8® reagents with different calcium-binding affinities (Fluo-8® Kd = 389 nM; Fluo-8H™ Kd = 232 nM; Fluo-8L™ Kd = 1.86 µM; Fluo-8FF™ Kd = 10 µM). We also offer versatile packing sizes to meet your special needs (e.g., 1 mg, 10x50 µg, 20x50 µg, and HTS packages) with no additional packaging charge.

警示图
危险性
危险性警示	No data available
安全声明	H319 (100%): Causes serious eye irritation [Warning Serious eye damage/eye irritation]
安全防护	P264, P280, P305+P351+P338, and P337+P313
备注

1. Liu L et al. TRPM7 promotes the epithelial-mesenchymal transition in ovarian cancer through the calcium-related PI3K / AKT oncogenic signaling. J Exp Clin Cancer Res 38:106 (2019).

2. Domínguez A et al. Effect of perfluorodecanoic acid on pig oocyte viability, intracellular calcium levels and gap junction intercellular communication during oocyte maturation in vitro. Toxicol In

3. Bender BJ et al. Structural Model of Ghrelin Bound to its G Protein-Coupled Receptor. Structure 27:537-544.e4 (2019).

4. Datta G et al. HIV-1 gp120 Promotes Lysosomal Exocytosis in Human Schwann Cells. Front Cell Neurosci 13:329 (2019).

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