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Hoechst 34580 可以被 405 nm 的紫色激光激发,并在约 440 nm 的发射最大值附近发射蓝色/青色荧光。 Hoechst 34580具有细胞渗透性
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HCR207321-50mg 50mg 97.8% ¥ 2850.00 ¥ 2850.00 2-3days
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HCR207321-10mg 10mg 97.8% ¥ 1580.00 ¥ 1580.00 880 2-3days
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中文别名 Hoechst 34580(911004-45-0),荧光染料
英文别名 Hoechst 34580(911004-45-0),Hoechst-34580,Hoechst34580
CAS号 911004-45-0
SMILES CN1CCN(CC1)c2ccc3c(c2)[nH]c(n3)c4ccc5c(c4)[nH]c(n5)c6ccc(cc6)N(C)C.Cl.Cl.Cl
Inchi InChI=1S/C27H29N7.3ClH/c1-32(2)20-7-4-18(5-8-20)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-9-21(17-25(23)31-27)34-14-12-33(3)13-15-34;;;/h4-11,16-17H,12-15H2,1-3H3,(H,28,30)(H,29,31);3*1H
InchiKey IPMZLKUUQSFUTA-UHFFFAOYSA-N
分子式 Formula C27H29N7. 3HCl
分子量 Molecular Weight 451.5733646
闪点 FP Not available
熔点 Melting point Not available
沸点 Boiling point Not available
Polarizability极化度 Not available
密度 Density Not available
蒸汽压 Vapor Pressure Not available
溶解度Solubility Soluble in H2O : 2.67 mg/mL
性状 Solid power
储藏条件 Storage conditions storage at -4℃ (1-2weeks), longer storage period at -20℃ (1-2years)

Hoechst 34580使用计算:

普通储备溶液的制备
Table 1. Volume of Water needed to reconstitute specific mass of Hoechst 34580 *CAS 911004-45-0* to given concentration.
Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

  0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 178.269 µL 891.345 µL 1.783 mL 8.913 mL 17.827 mL
5 mM 35.654 µL 178.269 µL 356.538 µL 1.783 mL 3.565 mL
10 mM 17.827 µL 89.135 µL 178.269 µL 891.345 µL 1.783 mL

The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Hoechst 34580 can be excited by violet laser at 405 nm, and emit blue/cyan fluorescence light around an emission maximum at ~440 nm. It is cell-permeable and provide an excellent tool for flow cytometers equiped with a 405 nm violet laser source.
Hoechst 34580(911004-45-0)实验注意事项:
1.实验前需戴好防护眼镜,穿戴防护服和口罩,佩戴手套,避免与皮肤接触。
2.实验过程中如遇到有毒或者刺激性物质及有害物质产生,必要时实验操作需要手套箱内完成以免对实验人员造成伤害
3.实验后产生的废弃物需分类存储,并交于专业生物废气物处理公司处理,以免造成环境污染
Experimental considerations:
1. Wear protective glasses, protective clothing and masks, gloves, and avoid contact with the skin during the experiment.
2. The waste generated after the experiment needs to be stored separately, and handed over to a professional biological waste gas treatment company to avoid environmental pollution.
产品说明 Hoechst 34580 (911004-45-0)可以被 405 nm 的紫色激光激发,并在约 440 nm 的发射最大值附近发射蓝色/青色荧光。 Hoechst 34580具有细胞渗透性
IntroductionHoechst 34580(911004-45-0):only use for non-medical purposes in industrial or scientific research, and not for clinical diagnosis and treatment of humans or animals. This product is not medicinal or edible.
Application1
Application2
Application3

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Authors: Zhang X, Kiechle FL.
Journal: Ann Clin Lab Sci (2006): 185


Usefulness of a triple fluorochrome combination Merocyanine 540/Yo-Pro 1/Hoechst 33342 in assessing membrane stability of viable frozen-thawed spermatozoa from Estonian Holstein AI bulls
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