WSP-5

使用方法
储存液制备：将低温保存的产品从冰箱取出，置于室温回温至少20min，低速离心3-5min之后内加入适量无水DMSO充分溶解成15M储存液(比如，1mg WSP-5(Mw: 591.7)加81ulDMSO)或更低浓度的储存液，请根据实际情况来调整，按照单次用量分装冻存，避免反复冻融，至少3个月稳定。
工作浓度：正式实验前，取15mM或其他浓度储存液用适当缓中液或培养基稀释到所需工作浓度即可，具体的工作浓度请参考文献或根据自身实需求来进行优化调整，参考下方些实验数据，来自公开文献，仅作参考
应用示例1: Hela细胞用DMEM+10% FBS培养2天，成像前一天，细胞转移到24孔内，检测前，贴壁细胞用FBS-free DMEM清洗一遍，对于胞内H2S成像，细胞用含WSP-5 (50uM) 的FBS-free DMEM于37℃孵育30min,用PBS (pH 7.4)清洗取出多余探针，然后细胞用溶于PBS bufer (pH 7.4,100uM CTAB) 的NaHS (100uM) 孵育30min，用PBS (pH 7.4) 清洗细胞3遍后，荧光显微成像 (490nm激发，绿色通道)。: Peng B et al Nucleophlic substitution-cyclization based fuorescent probes for hydrogen sulfide detection and bioimaging, Chemistry 2014Jan 20:20(4):1010-6.
应用示例2: SHSY5Y和U87MG用待研究的缓释天然硫化氢供体HBITC 40,60,80 uM) 或等体积含DMSO的培养基育，之后细胞用硫化氢探针WSP-5(50μM)CTAB (100μM in PBS)避光解育20min,用PBS清洗后,荧光显微成像检测，文献来源: Jurkowska H et al.New aspects ofantiproliferative activity oi4-hydroxybenzyl isothiocyanate, a natural H2S-donor Amino Acids. 2018 Jun;50(6):699-709.
案例：

?Fluorescence images of H 2 S in HeLa cells using WSP4 and WSP5. (a-f) Cells on 24-well plate were incubated with WSP4 (30 mm) for 30 min, then washed and subjected to different treatments: (a,b) Control (no NaHS was added); (c,d) treated with 30 mm NaHS; (e,f) treated with 60 mm NaHS. (g-l) Cells on 24-well plate were incubated with WSP5 (50 mm) for 30 min, then washed and subjected to different treatments: (g,h) Control (no NaHS was added); (i,j) treated with 50 mm NaHS; (k,l) treated with 100 mm NaHS. Scale bar: 100 nm.