HTPQA

---

---

HTPQA荧光探针 优点：
HTPQA荧光探针 是一种酶荧光成像探针，实现了活细胞内碱性磷酸酶活性的原位成像检测。该合成的碱性磷酸酶探针具有灵敏度高、选择性好等优点。该探针能够解决传统水溶性探针信号易扩散、衰减的问题，从而实现碱性磷酸酶的原位成像检测，除此之外，还成功应用于成像检测骨肉瘤细胞及组织中碱性磷酸酶的活性。这一新型固态发光成像探针的开发呈现出固态发光荧光染料在蛋白酶原位成像检测中的巨大优势。

---

Enzyme-responsive, fluorogenic probes fail to provide in situ information because the released fluorophores tend to diffuse away from the reaction sites. The problem of diffusive signal dilution can be addressed by designing a probe that upon enzyme conversion releases a fluorophore that precipitates. An excited-state intramolecular proton transfer (ESIPT)-based solid-state fluorophore HTPQ was developed that is strictly insoluble in water and emits intense fluorescence in the solid state, with λex/em=410/550 nm, thus making it far better suited to use with a commercial confocal microscope. HTPQ was further utilized in the design of an enzyme-responsive, fluorogenic probe (HTPQA), targeting alkaline phosphatase (ALP) as a model enzyme. HTPQA makes possible diffusion-resistant in situ detection of endogenous ALP in live cells. It was also employed in the visualizing of different levels of ALP in osteosarcoma cells and tissue, thus demonstrating its interest for the diagnosis of this type of cancer.

---

       目标探针HTPQA（红色信号通道）与商品化ALP探针4-MUP在HeLa细胞中共同孵育的成像图。从成像结果可以看出：（A, B）HTPQA能够实现ALP的原位检测，而4-MUP则扩散至整个细胞质；（C, D）HTPQA信号稳定，长时间监测信号无明显扩散，而4-MUP的信号逐渐消失。

---