MS培养基

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MS培养基成分mg / L：
MS培养基中的大量元素
硝酸铵1650.000
氯化钙332.200
硫酸镁180.690
硝酸钾1900.000
一元磷酸钾170.000
MS培养基中的微量元素：
硼酸               6.200
六水合氯化钴 0.025
五水合硫酸铜  0.025
EDTA二钠二水合物盐37.300
七水硫酸亚铁  27.800
一水硫酸锰     16.900
钼酸（钠盐）  0.213
碘化钾            0.830
七水合硫酸锌  8.600
MS培养基中的维生素：
肌醇                     100.000
烟酸（游离酸）     0.500
盐酸吡哆醇            0.500
盐酸硫胺素            0.100
MS培养基中的氨基酸 
甘氨酸                   2.000
总计（克/升）        4.4

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使用MS培养基注意事项：
•添加之前，确保培养基的pH合适胶凝剂，因为酸性pH值会导致胶凝减少产生半固体流动的凝胶，而碱性pH值会导致形成硬化的凝胶。
•使用蒸馏水/组织培养级水是建议用于自来水或更低的介质制备等级的水可能导致盐分沉淀和不合适凝胶化。
•避免制备浓缩液，因为这会导致沉淀盐。

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MS培养基使用指导：
•通过添加所需量的粉末占总体积的三分之二，保持恒定，温和搅拌直至培养基完全溶解。
•高压灭菌前添加热稳定剂
•用蒸馏水补足最终培养基所需的体积。
•使用1M NaOH溶液 /将培养基的pH调节至5.75±0.5盐酸。
•加入胶凝剂并将介质加热至沸腾胶凝剂完全溶解。
•通过在15 lbs和121°C下高压灭菌来灭菌培养基15分钟。
•添加热不稳定产品前，需将高压灭菌的介质冷却至约45°C热。
•在无菌条件下分配所需量的培养基无菌培养皿中的层流气流单元。

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MS Medium Description : Murashige and Skoog Medium (MS) was originally formulated by Murashige and Skoog in 1962 to optimize tobacco callus bioassay system for facilitating the study of cytokinins. Since then, it is widely used for micro propagation, organ culture, callus culture and suspension culture. The formulation is a nutrient blend of inorganic salts, vitamins and amino acid. Murashige and Skoog Medium (MS) provides all the essential macroelements and microelements. Potassium dihydrogen phosphate serves as a source of phosphate. Microelements like Boron, Manganese, Molybdenum, Copper and Zinc play vital role in plant metabolism. Boron plays a key role in carbohydrate metabolism. Thiamine, nicotinic acid, pyridoxine, inositol act as enzymatic cofactors in universal pathways including glycolysis and TCA cycle along with primary and secondary metabolism in the plants. Glycine serves as a source of amino acid.

Plant tissue culture for biotechnologyPrakash P. Kumar, Chiang Shiong Loh, in Plant Biotechnology and Agriculture, 2012.

Summary:Plant tissue culture involves excising plant tissues and growing them on nutrient media. It is used rather broadly to include several variations, such as meristem culture for propagation of virus-free plants, protoplast culture, cell suspension culture, tissue and organ culture, and anther or pollen culture for producing haploid plants. This chapter focuses on various technical aspects of plant tissue culture. A suitable explant is selected and prepared for culture, and later incubated on an appropriate nutrient medium for growth and differentiation. The basic laboratory setup, handling of explant tissue, nutrient medium and establishing the culture, and incubation of cultures are also discussed in this study. A laboratory that can handle plant biochemistry or physiology-type experiments meets most of the general requirements of plant tissue culture. It is a valuable tool for research on morphogenesis, cell signaling, physiology, and molecular biology, as well as crop improvement by biotechnology. The implications of plant tissue culture technology for agricultural biotechnology is immense.

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