LB培养基(液体)

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LB培养基的配方：

试剂	重量
Tryptone	10 grams
NaCl	10 grams
Yeast extract	5 grams
Distilled water	Up to 1 L

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LB培养基应用说明Application Notes
LB Medium is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. The ultimate in ease and convenience, LB Medium in a capsule form eliminates the waste, inaccuracies, and mess associated with weighing out bulk powder. The growth characteristics are the same as LB agar powder. LB Medium supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.

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LB培养基使用注意事项：
1.实验前需戴好防护眼镜，穿戴防护服和口罩，佩戴手套，避免与皮肤接触。
2.实验过程中如遇到有毒或者刺激性物质及有害物质产生，必要时实验操作需要手套箱内完成以免对实验人员造成伤害。
3.取样品的移液枪头需及时更换，必要时为避免交叉污染尽可能选择滤芯吸头。
4.称量药品时选用称量纸，并无风处取药和称量以免扬撒，试剂的容器使用前务必确保干净，并消毒。
5.取药品时尽量采用多个药勺分别使用，使用后清洗干净。6.
实验后产生的废弃物需分类存储，并交于专业生物废气物处理公司处理，以免造成环境污染。Experimental considerations:
1. Wear protective glasses, protective clothing and masks, gloves, and avoid contact with the skin during the experiment.
2. The waste generated after the experiment needs to be stored separately, and handed over to a professional biological waste gas treatment company to avoid environmental pollution.