Gly-Pro-AMC

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Gly-Pro-AMC 功能研究：
Gly-Pro-AMC is a sensitive fluorogenic substrate for detecting dipeptidyl peptidase IV (DPPIV), a serine protease that cleaves N-terminal dipeptides from polypeptides with L-proline or L-alanine at the penultimate position. DPPIV is a multifunctional protein expressed on the surface of several cell types including epithelial, endothelial and lymphoid cells. It is identical to the T cell activation antigen CD26 and the adenosine deaminase binding protein, and it is also released as a soluble form in plasma. The substrates of CD26/DPPIV include a wide variety of proline-containing peptides such as growth factors, chemokines, neuropeptides, and vasoactive peptides. DPPIV is involved in immune regulation, signal transduction, and apoptosis, and appears to play an important role in tumor progression. Importantly, DPPIV is a therapeutic target for type II diabetes due to its role as a serum protease that cleaves incretin hormones of the glucagon family of peptides and thus regulates glucose homeostasis. Studies indicate that a DPPIV inhibitor improves impaired glucose tolerance. The assay of DPPIV with Gly-Pro-AMC is a simple, fast and flexible assay that is ideal for high-through put screening. The assay demonstrates improved sensitivity compared to colorimetric DPPIV assays, allowing the researcher to use less enzyme while still achieving appropriate Z' values. The homogeneous coupled-enzyme format is convenient, requiring only a single reagent addition to the test samples. Maximal sensitivity is achieved in 20-30 minutes, and the signal generated is very stable.

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Gly-Pro-AMC存储液制备：
Gly-Pro-AMC 工作溶液：
※ 取出一个小瓶，用您选择的分析缓冲液（例如 50 mM Tris-HCl、pH 7.5、1.0 mM DTT）稀释储备溶液，制成 50 到 100 µM 的分析溶液。
※ 将特定质量Gly-Pro-AMC加入 DMSO 制备所需浓度体积的存储液。 请注意，仅用于制备储备溶液。 实验/缓冲液，请参阅示例实验协议。

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	225.545  µL	1.128 mL	2.255 mL	11.277 mL	22.555 mL
5 mM	45.109  µL	225.545  µL	451.091  µL	2.255 mL	4.511 mL
10 mM	22.555  µL	112.773  µL	225.545  µL	1.128 mL	2.255 mL

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Gly-Pro-AMC光谱图：

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Gly-Pro-AMC 实验注意事项：
1.实验前需戴好防护眼镜，穿戴防护服和口罩，佩戴手套，避免与皮肤接触。
2.实验过程中如遇到有毒或者刺激性物质及有害物质产生，必要时实验操作需要手套箱内完成以免对实验人员造成伤害
3.实验后产生的废弃物需分类存储，并交于专业生物废气物处理公司处理，以免造成环境污染Experimental considerations:
1. Wear protective glasses, protective clothing and masks, gloves, and avoid contact with the skin during the experiment.
2. The waste generated after the experiment needs to be stored separately, and handed over to a professional biological waste gas treatment company to avoid environmental pollution.