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1424150-38-8
  • Sulfo-Cyanine3 NHS Ester

  • names:

    Sulfo-Cyanine3 NHS Ester

  • CAS号:

    1424150-38-8

    MDL Number:
  • MF(分子式): C34H38N3KO10S2 MW(分子量): 735.8
  • EINECS:Not available Reaxys Number:No data available
  • Pubchem ID:154731801 Brand:BIOFOUNT
Sulfo-Cyanine3 NHS Ester
Sulfo-Cyanine3 NHS Ester(1424150-38-8)是一种有效的可以使用纯水溶液标记的蛋白质和肽,无需有机助溶剂, Sulfo-Cyanine3 NHS Ester 适合标记蛋白等对有机溶剂敏感的生物分子,也适用于溶解度低的蛋白质和易于变性的蛋白质。因为磺化Cy3极好水溶性特点,且染料分子本身带电荷,标记到蛋白表面后不会引发疏水性蛋白聚集,可以提高荧光标记产物的稳定性。ex/em (PBS): 550/566 nm
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中文别名 Sulfo-Cyanine3 NHS Ester;磺酸基Cy3活性酯:(1424150-38-8);磺化Cy3-NHS酯;Sulfo Cyanine3 NHS ester;磺化Cy3-NHS 活化酯;磺化Cy3-N-羟基琥珀酰亚胺酯
英文别名 1424150-38-8;Sulfo-Cyanine3 NHS Ester;Sulfo Cyanine3 NHS Ester;1424150-38-8 (sodium salt); 1424433-17-9, 1518643-34-9 (inner salt)
CAS号 1424150-38-8
SMILES CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC=C3C(C4=C(N3CCCCCC(=O)ON5C(=O)CCC5=O)C=CC(=C4)S(=O)(=O)[
Inchi InChI=1S/C34H39N3O10S2.K/c1-33(2)24-20-22(48(41,42)43)13-15-26(24)35(5)28(33)10-9-11-29-34(3,4)25-21-23(49(44,45)46)14-16-27(25)36(29)19-8-6-7-12-32(40)47-37-30(38)17-18-31(37)39;/h9-11,13-16,20-21H,6-8,12,17-19H2,1-5H3,(H-,41,42,43,44,45,46);/q;+1/p-1
InchiKey ZNWGQSGULWNMHO-UHFFFAOYSA-M
分子式 Formula C34H38N3KO10S2
分子量 Molecular Weight 735.8
闪点 FP Not available
熔点 Melting point Not available
沸点 Boiling point Not available
Polarizability极化度 Not available
密度 Density Not available
蒸汽压 Vapor Pressure Not available
溶解度Solubility
性状 solid,红色固体;dark red crystals
储藏条件 Storage conditions storage at -4℃ (1-2weeks), longer storage period at -20℃ (1-2years)

Sulfo-Cyanine3 NHS Ester说明:

A variety of cyanine dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy3® dyes are one type of the most common red fluorophores. These versatile fluorophores can tolerate a pH range of 3-10 for use in a variety of applications at biologically relevant pHs. The dyes are also DMSO tolerant and photostable to enable transfer from storage to assay without loss of performance. The aqueous solubility eliminates the need for organic solvents in the assay buffers. Sulfo-Cyanine 3 is an alternative dye primarily used for labeling peptides and oligos in replacing Cy3 that was originally developed by GE Healthcare (Cy3® is the trademark of GE Healthcare). NHS ester dyes are recommended for labeling amine groups and maleimide dyes are recommended for labeling thiol groups. This Cy3® NHS ester readily reacts with amino groups.
Sulfo-Cyanine3 NHS Ester 可替代:Cy3®、Alexa Fluor 546 和 DyLight 549

Molecular weight
735.19
Correction Factor (260 nm)
0.07
Correction Factor (280 nm)
0.073
Extinction coefficient (cm -1 M -1)
1500001
Excitation (nm)
555
Emission (nm)
569
Sulfo-Cyanine3 NHS Ester 实验注意事项:
1.实验前需戴好防护眼镜,穿戴防护服和口罩,佩戴手套,避免与皮肤接触。
2.实验过程中如遇到有毒或者刺激性物质及有害物质产生,必要时实验操作需要手套箱内完成以免对实验人员造成伤害
3.实验后产生的废弃物需分类存储,并交于专业生物废气物处理公司处理,以免造成环境污染Experimental considerations:
1. Wear protective glasses, protective clothing and masks, gloves, and avoid contact with the skin during the experiment.
2. The waste generated after the experiment needs to be stored separately, and handed over to a professional biological waste gas treatment company to avoid environmental pollution.
产品说明 Sulfo-Cyanine3 NHS Ester(1424150-38-8)是一种有效的可以使用纯水溶液标记的蛋白质和肽,无需有机助溶剂, Sulfo-Cyanine3 NHS Ester 适合标记蛋白等对有机溶剂敏感的生物分子,也适用于溶解度低的蛋白质和易于变性的蛋白质。
IntroductionWater soluble, amino-reactive sulfo-Cyanine3 NHS ester. Efficiently labels proteins and peptides in purely aqueous solution, without need for organic co-solvent. Ideal for proteins with low solubility
Application1
Application2
Application3
警示图
危险性 warning
危险性警示 Not available
安全声明 H303+H313+H333
安全防护 P264+P280+P305+P351+P338+P337+P313
备注 实验过程中防止吸入、食入,做好安全防护
1. Xu, D.; Wang, Y. smFRET study of rRNA dimerization at the peptidyl transfer center. Biophysical Chemistry, 2021, 277, 106657. doi: 10.1016/j.bpc.2021.106657
2. Taghian, T.; Metelev, V.G.; Zhang, S.; Bogdanov, A.A. Imaging NF-κB activity in a murine model of early stage diabetes. FASEB Journal, 2020, 34(1), 1198–1210. doi: 10.1096/fj.201801147r
3. Ni, C.-W.; Wei, Y.-J.; Shen, Y.-I.; Lee, I.-R. Long-Range Hairpin Slippage Reconfiguration Dynamics in Trinucleotide Repeat Sequences. The Journal of Physical Chemistry Letters, 2019, 10, 3985–3990
4. Cao, P.; Wei, X.; Awal, R.P.; Müller, R.; Wall, D. A Highly Polymorphic Receptor Governs Many Distinct Self-Recognition Types within the Myxococcales Order. mBio, 2019, 10, e02751-18.
5. Sallada, N.D.; Dunn, K.J.; Berger, B.W. A structural and functional role for disulfide bonds in a class II hydrophobin. Biochemistry, 2018, 57(5), 645–653. doi: 10.1021/acs.biochem.7b01166
6. Park, B.G.; Kim, Y.J.; Min, J.H.; Cheong, T.-C.; Nam, S.H.; Cho, N.-H.; Kim, Y.K.; Lee, K.B. Assessment of Cellular Uptake Efficiency According to Multiple Inhibitors of Fe3O4-Au Core-Shell Nanoparticles: Possibility to Control Specific Endocytosis in Colorectal Cancer Cells. Nanoscale Research Letters2020, 15, 165. doi: 10.1186/s11671-020-03395-w
7. Wang, C.; Fernández de Ávila, B.E.; Mundaca-Uribe, R.; Lopez-Ramirez, M.A.; Ramírez-Herrera, D.E.; Shukla, S.; Steinmetz, N.F.; Wang, J. Active Delivery of VLPs Promotes Anti-Tumor Activity in a Mouse Ovarian Tumor Model. Small2020, 16(20), e1907150. doi: 10.1002/smll.201907150
8. Saez Talens, V.; Arias-Alpizar, G.; Makurat, D.M.M.; Davis, J.; Bussmann, J.; Kros, A.; Kieltyka, R.E. Stab2-Mediated Clearance of Supramolecular Polymer Nanoparticles in Zebrafish Embryos. Biomacromolecules2020, 21(3), 1060–1068. doi: 10.1021/acs.biomac.9b01318
9. Choi, J.; Marks, J.; Zhang, J.; Chen, D.-H.; Wang, J.; Vázquez-Laslop, N.; Mankin, A.S:; Puglisi, J.D. Dynamics of the context-specific translation arrest by chloramphenicol and linezolid. Nature Chemical Biology2020, 16, 310–317. doi: 10.1038/s41589-019-0423-2
10. Song, P.; Shen, J.; Ye, D.; Dong, B.; Wang, F.; Pei, H.; Wang, J.; Shi, J.; Wang, L.; Xue, W.; Huang, Y.; Huang, G.; Zuo, X.; Fan, C. Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework. Nature Communications2020, 11, 838. doi: 10.1038/s41467-020-14664-8
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