钠离子指示剂介绍
钠离子指示剂允许在存在生理浓度的其他单价阳离子的情况下对 Na+ 浓度进行空间和时间分辨率。 这些钠指示剂在结合 Na+ 时表现出绿色荧光发射强度的增加，而波长几乎没有变化。

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钠离子指示剂
※ 测量 Na+ 浓度的稳态变化
※ 跟踪轴突和突触前末端的 Na+
指示剂可用于估计离体线粒体中的 Na+ 梯度、测量细胞内 Na+ 水平、测量细胞内 Na+ 流出，以及与其他荧光指示剂结合，将细胞内 Na+ 的变化与 Ca2+ 和 Mg2+ 浓度、细胞内 pH 值和膜相关联潜在的。

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钠指示剂选择指南

CoroNa Green	CoroNa Green, AM		SBFI, Tetraammonium Salt		SBFI, AM
Readout	Increase in green fluorescence emission intensity upon binding Na+, with little shift in wavelength		Spectral excitation shift used to estimate Na+聽gradients in isolated mitochondria and intracellular Na+聽levels
Range	Responds to a range of intracellular Na+around 10 mM		Responds to Na+in physiological range
Fluorophore	CoroNa Green		SBFI
Standard filter set	FITC		Requires excitation ratio to quantify Na+levels
Ex/Em (nm)	~492/516		~340 and 380/500
Bibliography	Citations		Citations
Cell permeant or impermeant?	Impermeant	Cell permeant	Impermeant	Cell permeant
Platforms	Imaging	Imaging	Imaging	Imaging
Usage notes	Load by microinjection, patch pipette, or pinocytic loading agent	Load in cell media	Load by microinjection, patch pipette, or pinocytic loading agent	Load in cell media
Format	1 mg	50ug	1 mg	1 mg	50ug
Cat. No.

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