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蛋白质和肽修饰试剂
Release Time：2022-09-14蛋白质和肽修饰试剂介绍：
蛋白质和肽的修饰试剂是用于修饰氨基酸侧链以改变天然电荷、阻断或暴露反应性结合位点、灭活官能团和改变官能团以产生交联和标记目标的化学试剂。
正确选择蛋白质和肽修饰试剂
1. 胺基蛋白质和肽修饰试剂
| 产品 | 功能 |
|---|---|
| Sulfo-NHS-Acetate | 胺反应性（NHS 酯）化合物永久阻断伯胺（例如，蛋白质赖氨酸侧链），以防止相互作用或随后的结合。 |
| Citraconic anhydride | 胺反应性化合物 (CAS 616-02-4) 可逆地用羧基封端赖氨酸和其他伯胺； 在 pH 7 稳定，但在 pH 3 至 4 时水解（解偶联）。 |
| TFCS | 胺反应性（NHS 酯）化合物； 末端的三氟乙酰基可以稍后去除以暴露出新的伯胺。 |
2. 异双官能团蛋白质和肽修饰试剂
| 产品 | 产品功能 |
|---|---|
| SATP | Short (4.1A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies. |
| SAT(PEG)4 | Long (18.2A), PEGylated form of SATA, an amine-reactive (NHS ester) sulfhydryl-addition reagent; contains 4-unit polyethylene glycol spacer arm. |
| SATA | SATA is a short-chain (2.8 angstrom spacer arm) reagent for covalent modification of primary amines and addition of a protected yet exposable sulfhydryl group, enabling heterobifunctional crosslinking strategies. |
| EMCA | EMCA is a sulfhydryl-reactive (maleimide) compound to permanently cap protein or peptide cysteines with carboxyl groups (make caproic acid derivatives). |
| AEDP | AEDP is an amine-and-carboxyl group containing compound that contains an internal disulfide bond; use with crosslinkers to modify functional groups on proteins and surfaces. |
| Traut's Reagent | 2-Iminothiolane-HCl (CAS 4781-83-3) is an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking. |
3. 巯基蛋白质和肽修饰试剂
| 产品 | 产品功能 |
|---|---|
| NEM | N-Ethylmalemide (NEM) is a small, sulfhydryl-reactive (maleimide) compound to permanently block the reduced cysteines of proteins or peptides to prevent disulfide bond formation. |
| MMTS | Methyl methanethiosulfonate (MMTS) (CAS 2949-92-0) reversibly block cysteines (sulfhydryl groups) to help facilitate the study of enzyme activation and protein function. |
| EMCA | EMCA is a sulfhydryl-reactive (maleimide) compound used to permanently cap protein or peptide cysteines with carboxyl groups (making caproic acid derivatives). |
4. 其他产品
| 产品 | 产品功能 |
|---|---|
| APTS | 3-Aminopropyltriethoxysilane (APTS)is a silylation reagent for coating glass and silica surfaces to add primary amines, which can be used to crosslink and immobilize proteins and other molecules. |
| NaIO4 | Sodium meta-periodate (NaIO4) (CAS 7790-28-5) is used to gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization. |
| IAA | Iodoacetic acid (IAA) is C2H3IO2 (CAS 64-69-7) is used to S-carboxymethylate sulfhydryls (reduced cysteines) at slightly alkaline pH for use in various protein biology methods. |
| Iodoacetamide | 2-Iodoacetamide (CAS 144-48-9) is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization and peptide mapping. Alkylation after cystine reduction results in the covalent addition of a carbamidomethyl group (57.07 Da) and prevents the formation of disulfide bonds. |
| Chloroacetamide | 2-Chloroacetamide (CAS 79-07-2) is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization. Alkylation with chloroacetamide after cysteine reduction results in the covalent addition of a carbamidomethyl group (57.07 Da) and prevents the formation of disulfide bonds. |
| Hydroxylamine-HCl | Hydroxylamine-HCl (NbOH.HCl) (CAS 7803-49-8) is used to cleave Asn-Gly peptide bonds or to deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent. |
| GlycoLink Coupling Catalyst | Aniline (CAS 62-53-3) and an optimized buffer is used to enhance aldehyde-hydrazide crosslinking efficiency for glycoprotein immobilization and conjugation. |
