7-Aminoactinomycin D (7-AAD)

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7-氨基放线菌素D (7-AAD)应用：
7-Aminoactinomycin D (7-AAD), an analog of the antibiotic actinomycin D (sc-200906), is a fluorescent DNA dye which is useful for quantifying apoptosis. 7-AAD has also been used in studies on cellular necrosis, chromosome analysis and in cell cycle studies. As a fluorescent DNA dye, 7-AAD is able to intercalate DNA via binding to the GC regions. When used in flow cytometery, 7-AAD can be used to discriminate between live cells as well as early and late apoptosis. Additionally, 7-AAD can also be combined with other staining procedures for analysis of intracellular and cell surface antigens enabling multiparametric analysis. When utilizing 7-AAD with flow cytometery, early and late apoptosis can be studied by varying the concentration of 7-AAD. During apoptosis, the cellular plasma membrane is altered progressively, which allows the cell membrane to become permeable to 7-AAD. 7-AAD staining can also be adapted to include staining with other dyes for intracellular and cell surface antigens. 7-Aminoactinomycin D is an inhibitor of DNA Polymerase and RNA Polymerase.

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制备储备液 for7-Aminoactinomycin D

以下数据基于产品分子量 1270.45。 由于水合程度的不同，批次的特定分子量可能因批次而异，这将影响制备储备溶液所需的溶剂体积。选择批次从而根据批次分子量重新计算: 

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
0.02 mM	39.36 mL	196.78 mL	393.56 mL
0.1 mM	7.87 mL	39.36 mL	78.71 mL
0.2 mM	3.94 mL	19.68 mL	39.36 mL
1 mM	0.79 mL	3.94 mL	7.87 mL

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7-氨基放线菌素D (7-AAD)光谱

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7-氨基放线菌素D 实验注意事项：
1.实验前需戴好防护眼镜，穿戴防护服和口罩，佩戴手套，避免与皮肤接触。
2.实验过程中如遇到有毒或者刺激性物质及有害物质产生，必要时实验操作需要手套箱内完成以免对实验人员造成伤害
3.实验后产生的废弃物需分类存储，并交于专业生物废气物处理公司处理，以免造成环境污染Experimental considerations:
1. Wear protective glasses, protective clothing and masks, gloves, and avoid contact with the skin during the experiment.
2. The waste generated after the experiment needs to be stored separately, and handed over to a professional biological waste gas treatment company to avoid environmental pollution.

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